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Of particular interest are hydroperoxide by-products, which may have escaped detection because of their lability, and unsaturated aldehydes.
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The previous lack of reports on the complete purification of GA 2-oxidases and GA 3-oxidases is probably due to a combination of their lability and their low abundance in most plant tissues.
In order to investigate whether the partial unfolding and/or 'breathing' motions leading to H-D exchange were part of the unfolding pathway of the protein we prepared a series of mutants, designed to produce cavities around the exchanging residue, and compared their rates of H-D exchange to their lability (rate of inactivation) in guanidine hydrochloride (Gd:HCl).
An even more dramatic demonstration of the correspondence of regions of extreme disorder and a lack of potential T cell epitopes will be discussed in Fig. 5. Taken together, these data suggest that disordered regions, probably because of their conformational flexibility, masking by nucleic acids and other proteins and their proteolytic lability, make poor antigens.
"Because of their age.
It's because of their greed".
This was because of their fanaticism".
Is that because of their race?
It was because of their skills".
Because of their personal financial problems".
Solids are inappropriate because of their inertness.
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