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However, this will not be the case for many other diploid animals or plants, including those attracting significant scientific interest because of their conservation status or usefulness.
Because of their conservation in the CTX-M family and the structural differences in this region compared to the TEM-type enzymes, we hypothesized that the two residues might interact cooperatively so that mutating both residues in the same molecule would have a larger effect on catalysis than expected from the individual mutations.
In most protected areas there are, in fact, focal species that have been selected for more detailed research and monitoring, e.g. because of their conservation status, because of their ecological relevance, because they are good representatives for a larger subset of species or because they are good indicators for certain habitat conditions [ 5, 65].
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"The people can no longer kill the lions because they understand the importance of their conservation for tourism".
Adults are variously reported to weigh between 900 and 2,300 kg (2,000 and 5,100 lb), although a study to collect accurate measurements of the animals has never been conducted and is not a priority because of their extreme conservation status.
NRs have been reported to be good phylogenetic markers, as they give robust results because of their structural conservation [ 12].
Because of their extreme conservation it has been postulated that these regions must have biological functions essential to mammal cells (2).
In addition, they may fail to recognize protein regions that are not associated to enzymatic domains because of their lower conservation rate.
In practice, the classification of mutations as drivers and passengers is based on their location at positions considered to be important because of their evolutionary conservation, and on observations in other experimental datasets (for a review of the methods used to classify driver mutations and the role of tumor progression models, see [ 74]).
On the demand side, growing appetites for oil in China, India and other developing nations have been offset by declining demand in the United States and Europe, because of their slowing economies, conservation efforts and growing use of biofuels.
But, such strategy was not suitable for minicircle derived RNA identification because of their lack significant conservation.
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