Once again, this is a bit surprising on the basis of the data presented in the previous sections, because in this direction of the titration the 3 1 protein DNA complex should become enriched.
For the titrations shown in the right panel (i.e., Kd ≅ 700 μM), the Kd value was calculated using only the initial part of the titration because the low solubility of MAC limited the measurements at higher concentrations.
Clear end points to the titrations were not obtained, as for wild-type FNR, because partial degradation of the [2Fe-2S] cluster during the course of the titration contributes to the Δ A420 readings.
Thus, the heat measurements in the competition assays correspond to the completion of the titration (i.e., the tail of the direct titration of panel B ) because 1.5 equivalents of CpxI(47-134) were not sufficient to saturate SCΔ60.
However, because the antimycin-insensitive component showed essentially the same amplitude over the range of the titration, the fractional contributions varied.
Potentiometric titrations involve the measurement of the potential difference between two electrodes of a cell; conductometric titrations, the electrical conductance or resistance; amperometric titrations, the electric current passing during the course of the titration; and coulometric titrations, the total quantity of electricity passed during the titration.
Note the biphasic appearance of the titration curve of SDS.
(C ) Isothermal titration calorimetry of the titration of His-tagged Nef (54 203) to the AP-2 α–σ2 hemicomplex.
On the other hand, the inability to maintain the blinding because the titration of insulin required monitoring of glucose levels may be a potential source of bias.
Second, our ST levels were determined by a somewhat crude method, because the titration protocol consisted of steps of ≥25 mC.
