This was partly because stocks of suitable vehicles were exhausted.
Because selection of suitable reference genes is important for qPCR assay, four commonly used reference genes, actin, EF-1α, EF-TU, and PP2A, were evaluated with geNorm [ 46], DeltaCT [ 47], BestKeeper [ 48], Normfinder [ 49], and RefFinder [ 50].
Although gibberellins and bikaverin are extracellular, it is not easy to select colonies after mutagenesis for enhanced gibberellins because of absence of suitable plate assays.
Many saproxylic insects (insects dependent on deadwood) are threatened because the amounts of suitable substrate, coarse woody debris (CWD), are low in managed forests.
We were unable to perform virus isolation studies because of lack of suitable biosafety infrastructure.
It is believed a third of British local authorities are in breach of the limit, largely because of a shortage of suitable temporary accommodation.
Half of those questioned said they waited longer than they wanted to move house, mainly because of a shortage of suitable properties.
More than half of American employers reported having difficulty filling positions because of a lack of suitable skills in 2010, one of the highest shares for developed countries.
At the beginning of the 19th century, sustained powered heavier-than-air flight remained an impossibility because of the lack of suitable power plants.
However, households have often been unhappy with choice-based schemes because of a lack of suitable homes of the right type in a good location.
