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Non-pharmacological possible causes of ALI were not present, because of stringent selection criteria (see Methods above).
In practice, some of them lack viable candidates because of stringent selection criteria, thus requiring the parameters to be loosened in order to identify additional candidates.
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Prospective teachers undergo a series of stringent selection processes including interviews and tests by the Ministry of Education MOEE) before they are selected.
The origin of these mutated B cells is unknown; the IgMIgDCD27 cells do not express AID and appear to acquire mutations independent of stringent selection by Ag.
Upon implementation of stringent selection strategies the frequency of targeting can be increased [ 18] even up to 100% [ 19, 20].
In GWAS, this bias is increased by the use of stringent selection thresholds and ranking large numbers of SNPs.
This emphasizes the need of stringent selection criteria and the validation of in silico identified SNPs via allele re-sequencing.
These experiments validate the laboratory use of stringent selection, which is essential to the practice of bacterial genetics.
The ability of stringent selection to prevent adaptation was demonstrated by Luria and Delbruck (1943) and Lederberg and Lederberg (1952).
In an earlier study, it was reported that the favorable characteristics of Pusa Basmati 1 with two BB resistance genes could be recovered using MAS just in BC1 because of stringent phenotypic selection without any background selection only in segregating generations (Joseph et al.2004).2004
In addition, we also performed a round of less stringent selection on these populations, selecting 25% closest to the desired level, and a round of more stringent selection, selecting only the 1% of the population closest to the desired level.
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