For example, SIH cannot infer haplotype regions which lack SNP fragments because of sequencing and mapping difficulties.
We focused on the areas surrounding the duplicated blocks rather than the duplicated region itself because of technical difficulties associated with sequencing a microsatellite-containing duplication.
Structures of tandem repeat sequences are difficult to accurately determine using data from whole-genome shotgun sequencing or next-generation sequencing, because of difficulty in assembling sequence reads into contigs.
However, where sequences were short or of low quality it was usually because of difficulty in sequencing through simple sequence repeats from the 5 'end of the cDNA clones or through mononucleotide stretches, such as poly A tails encountered in sequencing from the 3' end.
The resequenced regions included nearly all exons (except exon 11 of CYP3A7 because of difficulty in sequencing the highly homologous sequences) and relevant exon intron boundaries of four genes (CYP3A43, CYP3A4, CYP3A7, and CYP3A5) and two pseudogenes (CYP3AP1 and CYP3AP2) and approximately 2.5-kb promoter regions of the four intact genes.
Exon 1 of CERK was not sequenced because of technical difficulties.
No AD hybrid genomic sequence is currently available, mostly because of the difficulties of assembling the sequence reads into two genomes that share 85%to90%0% sequence identity.
This project may take considerable time, because of the difficulties involved in sequencing the huge (40 times larger than rice), highly-repetitive hexaploid genome of wheat.
This project may take a considerable time because of the difficulties involved in sequencing the large (40 times larger than rice), highly repetitive, hexaploid genome of wheat.
In fact, the challenging nature of bioinformatics prevents many biologists from embracing the new sequencing technologies because of the difficulties involved in analyzing the vast quantities of resulting data, which we refer to as the 'Genomic Deluge'.
