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The gene model shown in Figure 6A is based on B73 sequence information only because of sequencing constraints due to paralogs within the ZmALMT family.
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Other interconversions are unlikely because of sequence constraints.
The first 9,999 nt were selected because of constraints on sequence length by the programs used.
Because of resource constraints, we sequenced a minimum of 30% of the hMPV-positive samples from each month; 92 (45%) of 206 hMPV-positive samples were sequenced for the F gene, and 61 (30%) of 206 were sequenced for the G gene.
In an earlier study, it was proposed that ESS and protein interactions were negatively correlated with coding sequence ERs because of the constraints of important physical functions [ 3, 6, 11].
Amino acid sequences are generally more conserved than nucleotide sequences because of the functional constraints.
We focused on the fact that coding regions (CDSs) generally differ from non-coding regions by exhibiting a characteristic substitution pattern because of functional constraints on the protein sequences.
Thus, we can hypothesize that the 3'-terminal gene region might have a low propensity to accept new sequences or intron expansions, because of the constraints to preserve specific functional elements.
Furthermore, sequence constraints are incorporated.
For example, it is possible that the length of the coding and of the noncoding sequences are strongly related to each other because of structural constraints imposed by the process of splicing [18], such that the coding sequence length only has a relationship with expression because it is tightly tied to the gene noncoding sequence.
These findings are in agreement with those observed for other HEVs, for which recombination events in the capsid-encoding sequence are very rare, probably because of structural constraints that restrict the functioning of chimeric capsids [31].
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