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When images change nature rapidly because of sample damage [22], phase change [21] or though focus [24], taking the average of only the neighbouring frames and not the whole series is more appropriate.
aVNT, virus neutralization test; IFA, immunofluorescent antibody test; WB, Western blot test; NA, not assayed because of sample damage or loss during transportation or storage; CONT, contaminated samples that exhibited toxicity in VNT test.
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We identified three major steps of sample damage caused by the STED laser.
Examples of three major stages of sample damage: A – altered hemozoin structure, B – damages of fluorophores in the proximity of hemozoin, C – hemozoin disintegration.
First stages of sample damage, i.e. fluorophore bleaching, were observed only when the STED laser power exceeded 94 mW.
Sample damage in conventional or guided STED.
Guided STED prevented sample damage by switching off the STED laser in areas with high amounts of hemozoin.
These 20 samples were chosen because of sample abundance.
We did not capture obviously abnormal guard cells with artifacts because of mechanical damage in sample preparation.
That's because of sampling theory.
It appears that the reason for not observing improvement in fracture toughness of CNT-reinforced samples is because of either damage to CNTs or possibly non-optimal interfacial bonding between CNT-alumina.
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