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This is especially the case for homogeneous immunoassays and surface measurements on tissue sections and membranes, which typically have a high background because of sample autofluorescence.
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This method will also decrease the contribution of sample autofluorescence to the detected signal.
Figure 7 shows fluorescent images of QD_CHI0.67_3.5 internalization in comparison with the control sample (autofluorescence).
Topotecan uptake was corrected for control sample autofluorescence.
Also with the use of biological samples, autofluorescence and back scattering light from paper surfaces can be even more problematic [84, 85].
That's because of sampling theory.
Chloroplasts appear red because of autofluorescence.
In that latter case, LOFLO nutritive medium (Formedium) was used because of a reduced autofluorescence compared to the original medium.
As hypericin fluoresces in the orange red region (emission peak about 590 nm), the green fluorescence emitted is most likely because of autofluorescence of the tissue.
Because of the intrinsic autofluorescence problem in the living animals, the egfp-harboring TF gene constructs were not chosen for in vivo imaging applications.
Without complexities of sample preparation and potential toxicity of exogenous biomarkers, autofluorescence imaging of endogenous fluorophores promises a noninvasive method for functional measurement of the retina [ 13].
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