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We suspect that the high number of unassignable sequences from genomic DNA is because of recombinants that were created during PCR because of an excess of potential recombination partners present in the genomic DNA, which is in contrast to the situation in cDNA where much less such recombination partners are present.
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Because approximately 50% of recombinants were observed for γ2 env, with even some apparent inter-group recombinants detected by the PHI-NNet algorithm, the non-parametric test was re-run using only the non-recombinant dataset.
Again, this seems unlikely because addition of recombinant RAGE to sputum samples did not affect measured levels compared with controls.
Both tests were carried out because presence of recombinant sites in the nuclear genes leads to a loss of power to reject neutrality [ 60].
Because we excluded the possibility of recombinants when filtering alleles used in this analysis (see Materials and Methods), the effect of recombination on the KB(m) accumulation pattern is quite small.
Because this algorithm identified a smaller number of recombinants, we used the observed non-recombinant dataset in our phylogenetic reconstructions.
Use of more advantageous protein expression systems is necessary for plant-based human-like glycoprotein production [reviewed in [ 33]] because the yield of recombinant proteins is very low and because expensive purification steps are required.
It must be noted that the observed fraction of recombinants may differ from the actual fraction of recombinants because multiple COs occurring between two markers often remain undetected.
Modulation of wound healing because of administration of recombinant EPO or inhibition of endogenous EPO-EPO receptor correlated with changes in levels of inducible nitric oxide synthase protein in granulation tissue.
These measures have greatly reduced the threat of pathogen transmission, but some risk remains because a number of recombinant products still incorporate plasma-derived additives during manufacture [ 19].
Utilizing SNPs identified in the present study, expansin was ruled out as a candidate gene for Sr2 resistance because re-examination of recombinant Chinese Spring-Hope lines studied by Kota et al [ 34] identified a recombination event between the Sr2 phenotype and TaEXPB11hp (R. Mago unpublished).
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