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Although the role of AMPK in regulating protein synthesis is generally believed to be negative, AMPKα1 and α2 null embryos are lethal ~10.5 days post-conception because of poor expression of genes important for growth and survival [ 45].
Because of poor expression and low protein yields for RcPutAE1012A, 12 L of LB broth was used for expression, 0.2% Triton X-100 was included in the sonication buffer, and the ion exchange step was omitted.
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Transduction of human mesenchymal stem cells (MSCs) with type 5 adenoviruses (Ad5) is limited in the efficacy because of the poor expression level of the coxsackie adenovirus receptor (CAR) molecules.
Application of assisted reproductive technologies in buffaloes is limited to some extent by farmers' inability to detect oestrus because of its poor expression.
Some of these clones, however, may not be functional as a result of the cloning steps (stop codons present in the oligonucleotides and PCR) or because of a poor expression level in the bacterial cytoplasm.
There is also a possibility that the His-tag was not detectable in the other clones because of their poor expression levels, but this has not been explored further.
The deal got postponed because of poor market conditions.
Physical analysis of the components of the telomerase complex has been hampered by technical challenges because of low natural abundance, poor expression, and inefficient in vitro assembly of the recombinant enzyme.
But, because of his inchoate love for the girl, his poor expression of his own grief, he is unable to understand that need.
We speculated that the polymorphisms of ZNF665 might lead to poor gene expression because of poor DNA binding ability, which might disturb cell growth and differentiation; in turn, this disturbance might impede epithelial repair and lead to the dry, cracked, scaly, or tough skin that is characteristic of patients with the YZ constitution.
One possibility is that the expression differences observed in certain transcripts could be because of poor hybridization due to sequence differences rather than genuine expression differences.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com