Two specific genes, AtMRS2-10 AtSULTR1 2R1;2, were chosen for this study, in part because of their localization and because of availability of knockout lines.
To exclude the possible compensatory action produced by Kir6.1 because of Kir6.2 knockout, expression of Kir6.1 subunits was determined in Kir6.2−/− mice.
Because of OCRL knockout mouse did not exhibit a phenotype, we examined the function of ocrl in motile cilia of zebrafish and presented data to show decreased cilia numbers in the KVs in ocrl morphant animals.
The similar protein levels of Kir6.1 subunits in the left ventricle between WT and Kir6.2−/− mice suggested that no compensatory increase happened for Kir6.1 subunits in the heart because of Kir6.2 knockout.
Hypoesthesia has been reported to be dependent on the expression of AT2R, because genetic knockout of this gene resulted in restoration of normal latency period for sensing of pain from 11 to 8 s (Marion et al., 2014).
ORE1/NAC2 was genetically identified as a positive regulator of leaf senescence, because knockout of ORE1/NAC2 extends plant longevity in Arabidopsis [ 25], and miR164 mediates the cleavage of a group of NAC family genes, of which ORE1/NAC2 is a positive regulator of aging-induced cell death and leaf senescence [ 25, 31].
It is unnecessary to test such a pair, because a knockout of { r, s} is equivalent to the single knockout of r, see Figure 1b) for illustration.
About half the menu consists of knockouts.
Because the knockout of Sec61β was reported to lower protein secretion in S. cerevisiae, but not in Y. lipolytica [23], [27], we wanted to assess the importance of SP_Sec61β in protein secretion.
Golovkin has become one of the most talked-about fighters in the world because of a 90% knockout percentage that belies his sweet personality.
