In this study, because the number of clinical samples was small, further studies are required to explore the relationship between the SBSN expression and clinical background in larger numbers of patients.
RRBS is the first choice for DNA methylation analysis of clinical samples because of its minimal DNA requirements and low cost, based on reduced representation of the genome.
Because of the limited availability of clinical samples, fluorescence-based methods, such as laser confocal microscopy or fluorescence resonance energy transfer, are preferable.
Because large-scale whole-genome sequencing of clinical samples is not yet feasible, multilocus sequence typing (MLST) for C. trachomatis has been developed to provide greater insight into strain types; 3 such MLST methods have been reported in the literature (5 – 10 ).
This higher prevalence of ESBL and AmpC by Altun et al. may be because of the selection of various types of clinical samples from the ICU patients.
Clearly more work with community samples of preschoolers, preferably in more countries, needs to be conducted before one can conclude whether the propensity to distort because of depression is mainly a characteristic of clinical samples.
Preservation of clinical samples collected daily is an essential undertaking, because each sample is unique and has the potential to facilitate future research and diagnostics.
Because persons who died were not sampled postmortem, only a limited number of clinical samples were collected (Table 1).
However, because clinical samples are typically subject to higher rates of comorbidity, studies of clinical samples may have exaggerated the degree of the temporal relations between ODD and CD [ 13].
Among them, 40 were excluded from analysis because they were diagnosed as having secondary HLH, or their parents did not provide permission for use of clinical samples.
