Alternatively, because 20 times more sequences are available than for EBOV, we selected years with at least ten strains of one category to reduce the artefactual effects derived from sequencing uncertainty.
Ten segments were excluded from phylogenetic analyses after visual examination of each alignment because it was obvious that one or more sequences were not homologous.
In case of QV1, the number of large clusters increases, because more sequences can be assigned to clusters for greater QV1 values.
Finally, we compare the genome sequence assembled using our hybrid strategy to a classical reference assembly using the same data as input and show that with the availability of a reference genome, it pays off to use the hybrid de novo strategy, rather than a classical reference assembly, because more genome sequences are preserved using the former.
Because 16S rRNA sequencing is more cost-effective than whole metagenome shotgun sequencing, marker gene analysis is frequently used for broad studies that involve a large number of different samples.
Because partial rpoB sequencing is more discriminatory than 16S rDNA sequencing, a cutoff value of ≤95% similarity proved suitable for species identification within Corynebacterium (12 ) and also clearly enabled species identification in this study.
Because more genomic sequences are now available, we can also expand upon these earlier findings by more accurately defining the phylogenetic distribution of G-rich intronic sequences.
Because sequencing is currently usually more expensive than sampling of individuals, this clearly implies that there is little advantage to sequencing at a depth of coverage much greater than the numbers of individuals in the pool provided is on the order of N or smaller, essentially every sequence will be derived from a different chromosome.
An identifier is only established if the peptidase in question can be shown to differ from an existing peptidase because there is a significant difference in the action on one or more substrates or in the interaction with one or more inhibitors, or, more likely, because the sequence is too divergent to be included within an existing MEROPS identifier.
(Exome sequencing is more comprehensive than 23andMe's SNP tests, but still isn't as exhaustive as whole genome sequencing).
