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We chose this cell line because it expresses low levels of endogenous Tiam1, and we had previously shown that overexpression of activated Rac is sufficient to induce a motile phenotype [ 50].
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Yet is this picture popular because it expresses the truth, or because it softens it?
These macrophages, however, do not represent true M2a macrophages, because they express low levels of MR and Dectin-1.
We took advantage of PC12 cells because they express low levels of ZnT3, are easily transfected with tagged versions of this transporter, and ZnT3 endosome/synaptic-like microvesicles (SLMVs) subcellular localization is well characterized in these cells [26].
It is not clear whether this activity originated from expression in endocrine progenitors in the gut, or persistent expression in the pancreas, because adult islets express low but consistently detectable levels of neurogenin-3 mRNA (Fig. 1B) (Wang et al., 2009).
And the letter to Governor Stevenson: "The general won because he expressed low-grade universal potato love".
Because androgen-responsive, non-metastatic LNCaP cells expressed low levels of RANKL, we tested whether stable, forced RANKL expression in LNCaP (LNRANKL) cells promoted RANKL RANK signaling and bone metastasis.
To evaluate the hypothesis that C. elegans hermaphrodites express low mating frequency because they have the ability to self-reproduce without males, we depleted hermaphrodite self-sperm and measured mating frequency.
We did not to make the claim that let-7 overexpression is effective in MYC cancers solely because it is expressed at low doses.
Missense mutant pVHLs downregulated the promoter activity of HRE as well as the mRNA levels of Glut1 (Fig. 5c) and VEGF (Fig. 5d); however, L158Q showed little effect on the HRE promoter because it was expressed at a low level.
We do not have the molecular explanation for the lack of IFNα production but it is known that monocyte-derived DC are weak producers of IFNα in response to virus or TLR stimulation while they produce substantial amounts of IFNβ, probably because the express low levels of interferon regulatory factor 7 (IRF-7) [51].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com