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Among the genes, we focused on the MDA-7/IL-24 gene because it encodes an anticancer cytokine (Dash et al, 2010).
GI-23 is noteworthy because it encodes an EmrKY-TolC multidrug resistance efflux pump and the sensor kinase, EvgA, involved in acid resistance and multidrug resistance in E. coli [ 32].
The PA gene was chosen as a control to the HA because it encodes an internal viral protein and is among the viral genes that are the least likely to be subject to substantial positive selection.
The ESR1 gene is critical for hormone binding, deoxyribonucleic acid binding, and activation of transcription, because it encodes an estrogen receptor that is key in the mediation of hormonal response in estrogen-sensitive tissues.
We paid special attention to the gene agl1, not only because it is the only gene affected by a LoF mutation within the 200-kb region but also because it encodes an extracellular alpha-glucosidase involved in maltose utilization (Kato et al. 2013).
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YAP1 is another interesting candidate for industrial strain engineering because it encodes a transcription factor (Yap1p) that simultaneously controls a wide range of stress-related targets (Toone and Jones [1999]).
The DNA sequence of dardarin is interesting because it encodes a protein that looks like a kinase, which is an enzyme that dabs phosphate groups onto proteins to allow them to communicate with each other.
PVS may be of relevance to myotonic dystrophy (DM) and the inherited motor neuron diseases: to DM because it localizes to the same region of chromosome 19 and to the inherited motor neuron diseases because it encodes a cell-surface receptor expressed on motor neurons.
We suspect LeBron is a temperate phage because it encodes a tyrosine-integrase (gp36), although no homologues of known phage repressors are evident.
It was chosen as our 3' reporter gene for this system because it encodes a Class I antigen presentation molecule that, like the Dad1 gene, can be expressed by most cell types.
ARG81, located in the QTL region B, is a relevant candidate gene because it encodes a transcription factor that regulates gene expression in response to arginine availability.
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