Because our gene expression analyses had demonstrated strong intestinal expression of genes potentially involved in the zebrafish Nod signaling pathway, we utilized a transgenic ifabp GFP line that expresses GFP within intestinal epithelial cells to monitor gut development.
Because of single-cell gene expression analyses, normalization to the housekeeping genes was not performed.
Gene expression analyses of the genes mentioned above were performed by quantitative PCR.
Although the in vitro DST suffers from difficulties in duplicating the complex conditions of in vivo therapy, they would have some advantages over the gene expression analyses because numerous known and unknown molecular networks influence the susceptibility of the tumour cells to anticancer agents.
Even though these various fat depots are differentially responsive to a number of stimuli (e.g. diet), and are thought to make specific contributions to glucose and metabolic homeostasis [ 26], we chose to focus on epididymal WAT and interscapular BAT pads for histological and gene expression analyses because they could be reproducibly isolated from knockout mice.
In fact, luminal Ab resemble luminal B tumours; they are distinguished only because the lists of genes used in gene expression analyses to identify subtypes are not accurate enough and because luminality reflects a continuum from poorly differentiated, highly proliferative (luminal B) to well-differentiated, poorly proliferative (luminal Aa).
Gene expression analyses are important for the revealing of gene functions.
This redundancy complicates downstream applications of the resulting transcriptome, such as gene expression analyses [ 30], because reads from some single copy genes will not map uniquely within the transcriptome and may be discarded.
Next, to investigate any rescue because of the recovery of P-bodies, we conducted gene expression analyses of male gonads from these embryos at E15.5.
Because of the complexity of evolution it becomes necessary to broaden gene expression analyses to the full set of paralogous genes.
