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We chose to use 10 μmol/l nutlin-3 because at this concentration nutlin-3 alone induced little cell death (<4% of U-2 OS cells died after 72 hours).
For the experiments in this study we decided to use 100 n m, because at this concentration mTORC1 is efficiently blocked and rapamycin mediates its well-known cell cycle effects with only minor effects on Akt phosphorylation (Fig. 1A C).
In this regard, Rhombeobin (0.1 μg/animal) activates both the intrinsic and extrinsic coagulation pathways and 5(d)), but it does not induce defibrinogenation) because, at this concentration, Rhombeobin did not effectively degrade fibrinogen).
mL−1 (3.0 as00) ad ad libitum (AL) feeding, because at this concentration wild-type (WT) lifespan most closely resembled published observations on plates and in liquid (Fig. 1B,C, Table S3).
The concentration of Ca2+ was set at 150 μM because at this concentration there is a molar excess of calcium in the subphase with respect to lipid and protein concentrations.
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2 μg/mL was specifically chosen because, at that concentration, CisPt resulted in a low cell death percentage (~20%) which would allow the relative influence of PBMCs and/or mechanical stress to be determined during experimentation.
We used 10−9 M DPI because DPI at this concentration is effective at inhibiting PHOX activity without any observable neuro-toxicity (Qin et al. 2002).
The aqueous NaOH treatment of all four cellulose samples was conducted with 16.5 weight percent aqueous NaOH because treatment at this concentration has been reported to cause complete conversion of cellulose I to II [ 29].
The mutagenicity of the highest iCNSL concentration (69.50 µg·mL−1) was not confirmed when analysing the frequency of chromosomal aberration (CA) because iCNSL at this concentration had already been shown to have mutagenic effects as evidenced by increased MN frequency, and MI inhibition also precluded observation of CA at this concentration.
This is because, at higher concentration, the active sites of the adsorbent get saturated very quickly (Mondal et al. 2013).
Absent a subculture concentration standard, we applied rifampin at 1 mg/L (4 ) because bacteria growing at this concentration are considered resistant to rifampin.
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