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Oocytes became nonviable with clearly resolved granulated cytoplasm following incubation with undiluted polymeric first wash medium.
By E9.5, however, EpCAM knockout embryos were smaller than EpCAM sufficient embryos, neural tube closure was often delayed, and all EpCAM knockout embryos became nonviable before E12.5.
Examination of embryos of various gestational ages revealed that EpCAM −/− embryos appeared to develop normally up to E8.5, after which development arrested, and became nonviable during E10.5-E11.5 (Table 3).
Strains isolated from patients 1, 4, and 5 became nonviable during storage in the hospital laboratory.
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Rainfed cropping, already marginal in terms of viability, will likely become nonviable and will be almost eliminated.
Although spores survive outside the host, they become nonviable when dried at room temperature for 5 days (30, 31 ).
In a member of this family, named the gef gene (isolated from the chromosome of E. coli) (Poulsen et al, 1991), the killing function is mediated by a membrane protein of about 50 amino acids, which induces the arrest of cellular respiration; subsequently, the cells undergo morphological changes (characteristic 'ghost' cells) and become nonviable (Poulsen et al, 1992).
The Wichita Foundation became financially nonviable after a court ruled that it was liable for the unpaid debts of its defunct predecessor in Elizabeth, N.J.
But it was their potential use in humans that riveted the public and policy makers this year after a Chinese team became the first to publicly report using it to alter the DNA of human embryos (nonviable ones produced as part of IVF efforts) and a U.K. group said it wanted to do similar research in the United Kingdom.
Instead, they pooled cells from viable embryos from women in whom all transferred blastocysts developed into babies, and from nonviable embryos from women who did not become pregnant but had no known uterine deficiencies.
The consequences of this (in our model) are important: 1) Viable individuals evolve to increase the fraction of lethal (nonviable) sequences in their mutational neighborhood, with the latter acquiring novel functionalities; and 2) the exploitation of nonviable mutants by units of replication to outcompete other viable individuals becomes more extreme the higher the mutation rates.
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