Exact(3)
Distinctive features are the paseo ("walk"), bien parado ("sudden stop"), and various beating steps (battements).
Sure, there are plies, kick ball changes, beating steps and an intense one-hour full-body warmup.
Frozen colon contents were thawed, and bacterial genomic DNA was extracted using QIAamp DNA Stool Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions, with the additional glass-bead beating steps on a Precellys 24 homogenizer (Bertin Technologies, Montigny, France).
Similar(57)
DNA Prep #3 (Enz): Identical protocol to DNA Prep#1 but without the bead beating step.
Finally, to assess DNA extraction efficiency for each organism in isolation, an enzymatic extraction with a bead beating step (EnzBB) was applied to each isolate culture separately.
We isolated DNA from lung tissue samples by the same procedure, except that the duration of each bead beating step was increased to 2 minutes.
For the pellet fraction, an additional bead beating step was included.
Phenol chloroform extraction generally followed the procedure proposed by Maciel et al. (2009) including a lysozyme treatment (2.5 mg ml−1) and a beat beating step (beat diameter 1 mm, 10 min beating time).
RNA was isolated essentially as described previously [ 26], except that an additional bead beating step was included for efficient Gram-positive cell lysis.
Freeze-dried cells were disrupted by a 30 min bead beating step in the presence of a chloroform:methanol mixture (1 1.25) to extract the lipids from the biomass.
Total RNA was purified from each sample using the standard TRIzol (Invitrogen) extraction protocol, with the addition of a bead beating step (three times for 20 s at 5 m/s, with 10 mins incubation on ice between beating).
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