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The quintessential property of developing cardiomyocytes is their ability to beat spontaneously.
The cardiomyocytes in the cell sheets beat spontaneously and synchronously, and expressed connexin 43 at the edge of adjacent cardiomyocytes.
In this study using the isolated cardiac cells we also fabricated a cardiac cell sheet that beat spontaneously and synchronously.
All graft regions exhibited cyclic changes in fluorescent intensity that occurred synchronously in a 1 1 relationship with the host ECG when the heart beat spontaneously or was electrically paced at rates from 3too 5 Hz.
The hearts were allowed to beat spontaneously.
Control cultures beat spontaneously with a mean rate of 80.4±5.8 bpm (n = 17).
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Moreover, they started beating spontaneously within the first 24 hr of culture (Supplementary Videos 1–3).
After 18 h myocytes were confluent and beating spontaneously.
After 18 h, myocytes were confluent and beating spontaneously.
Unipolar electrogram recordings were made from hearts beating spontaneously.
A unipolar electrogram was recorded from each channel with hearts beating spontaneously.
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