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An advantage of the beat method of estimating optimal drive sweep rates for maximal autoresonant response is that no model is required so experimentally generated beat oscillation data can be used for systems where no model is available.
Pressure recording analytical method (PRAM) is an invasive continuous (beat to beat) method based on the analysis of the morphology of both the pulsatile and continuous components of the arterial pressure waveform, at a higher sampling frequency, than the other pulse contour analysis technologies [19].
However, the single beat method has been well validated against conductance catheters and provides reproducible results [ 19].
Despite several proposed non-invasive approaches to evaluate Ea/Ees, only the modified single beat method developed by Chen et al. has been validated against the invasive measurement of Ees [ 23].
The single beat method is based on the assumption that because the time-variation of LV elastance is not influenced by loading conditions or heart rate (HR), the evaluation of the P-V loop can be obtained by a single heart beat [ 24].
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This phenomenon was also seen consistently when the bead-beating method was applied to the cells (Krause et al. 2004; Villegas-Rivera et al. 2013).
Extraction was continued as per bead beating method.
Extraction was then continued as per bead beating method.
When the DNA extraction methods were compared (Table 2), the bead beating method consistently extracted DNA with higher 260/230 and 260/280 ratios.
The bead beating method used here performed better than those previously reported which usually extract DNA of less than 10 kb in size (3).
Total DNA of the biofilms samples was extracted and purified using a bead beating method (FastDNATMSPIN Kit for Soil, Bio101 Inc., USA) following the manufacturer's instructions.
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