"It's definitely contagious". That's good, because the conservative beat itself is not at full speed at the moment.
We added approximately 160 mg of 0.1 mm zirconia beads, subjected them to beating for 20 s at full speed, and incubated them at 56°C for 30 min. We then spinned down beads at 5000 g for 30 s, transferred supernatant to a new tube and continued with peqGOLD Tissue DNA Mini Kit (Peqlab, Erlangen, Germany).
And, by the way, while I'm at it, can I point out that David (who, in the interest of full disclosure, I run with sometimes) has a particularly high reverse Kipsang number — the reverse Kipsang being the maximum distance you could run, at full speed, and beat Wilson Kipsang at full speed.
And, by the way, while I'm at it, can I point out that David (who, in the interest of full disclosure, I run with sometimes) has a particularly high reverse Kipsang number the reverse Kipsang being the maximum distance you could run, at full speed, and beat Wilson Kipsang at full speed.
Subsequently, 40 µl of proteinase K was added to each sample, which was then incubated for 10 minutes at 65°, followed by a second bead-beating for 1 minute, then centrifugation at full speed for 1 minute.
