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The homozygous lethal line KS030 bears an insertion in an intron of lozenge.
The PLDδa protein bears an insertion of 11 aa in an otherwise relatively conserved region, which could have profound functional consequences.
In addition, for human CLK1 and CLK3 (PDB: 1X57 and 2EU9, respectively), the C-terminal lobe bears an insertion between the two sheets β7 and β8, termed the CLK-specific βhp-βhp' hairpin, and renders the substrate docking groove inaccessible (as compared to the substrates of MAPK which are able to access a similar groove) [ 50].
Similar(57)
Southern blot analysis confirmed that each HSV-2 mutant virus bore an insertion of GFP coding sequence in place of a deletion of the expected size in the ICP0 gene (Fig. 1A, 1C).
The resulting plasmid, p0+GFP, encoded the ICP0+GFP reporter protein, which bears a GFP insertion between amino acids 104 and 105 of ICP0.
We also compared the ability of nucleic acids to stimulate the ATPase activity of the human DDX1 protein, that bears a long insertion (250 aa) between motifs I and Ia, but shows no similarity within this region with the equivalent region of DDX3.
For example, the derived allele Dpul_341016 at the Dpul_341016 locus bears a single insertion in a coding region that is homologous to exon 2 at this locus in all other D. pulex clones and outgroup species.
Such a critical role for TMD1 could help to explain the loss of function mutation of Slc11a2 construct 1, which bears an HA tag insertion immediately C-terminal to TMD1.
This last mutant, designated 1-5NC-S21 1-5NC-S21 1-5NC-S21de insertion in the loop of a stabearsNa hairpin at the very 5' end of the viral genome.
It is interesting to note that the human protein DDX1, which bears a very large insertion (250 aa) between motifs I and Ia, has a clear preference for RNA as an activator for its ATPase and does not show DNA helicase activity (Fig. 4 A and 6 B).
br04 bears a single nucleotide insertion in At1g55490, which encodes a plastid chaperonin 60β (ptCpn60β1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com