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Inoculum was prepared by re-suspending bacterial culture in sterile, distilled water at an optical density of 0.2 (DO600), bearing approximately 108 cfu per ml.
Each single RNA sample was subjected to microarray data analysis on Affymetrix DNA chips bearing approximately 22,000 probe sets, which collectively interrogate 14,500 human genes.
Then, aliquots of 50 μl of extracts were added to two discs bearing approximately 100 preconditioned seeds and incubated at 25°C.
These 28 samples were hybridized on whole-genome oligonucleotide microarrays bearing approximately 44,000 probes, representing 25,585 nonredundant genes with gene symbols [ 21] (Additional file 1).
During the last 2 decades molecular genetic research has lead to the identification of five important PD genes bearing approximately 500 different DNA variants.
Six branches, each bearing approximately 60 leaves, were cut 4-5 m above ground level, in a transect from east to west across the canopy, or the nearest feasible positions, for morphological and herbivore community analyses.
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A similar phenomenon was observed by Bernhardt et al. They indicated that supporting the arms on the standing frame tray while standing reduced the weight bearing by approximately 10% body weight.
4 out of 5 mice bearing large tumors (approximately 200 mm3 before treatment) were cured by 4D5-BBZ CAR-transduced OKT3-28BB-86BBL RNA-T cells, and 5 out of 5 were cured by CD3/CD28 Dynabead 4D5-BBZ CART cells (Fig. 6D).
Thakur et al. (2003) showed methods and materials for experiments in nude mice bearing human tumors: approximately 5 × 106 viable human prostate (DU145), breast (T47D), or colorectal cancer (LS174T) cells were implanted into nude mice in groups of ten mice each, and tumors were allowed to grow to (0.61 cm in diameter) 5 × 108 ng and treated with 16.7 MBq (450 μCi).
Because antiangiogenesis therapy is likely to be most effective in a low tumour burden state (Bergers et al, 1999), we started the treatment in mice bearing tumours ranging approximately from 25to4848 mm.
For the evaluation of tumour necrosis, mice bearing tumours of approximately 450 mg were randomised and treated with vehicle or PTX (20 mg kg−1, i.v ., followed 2, 24, 72 h, and 1 week later by ZD6126 (200 mg kg−1, i.p).
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