Sentence examples for bearing a vector from inspiring English sources

The growth rates of wild-type and rad23Δ dsk2Δ mutant cells bearing a vector plasmid are similar under the condition.

Here, we directly tested the requirement for Drp1 in cell death induced by activation of RIPK3 by using Drp1 −/− MEFs bearing a 4HT-inducible vencodingcoding a RIPK3-gyrase fusion protein that dimerizes in response to coumermycin.

Competition experiments have to be controlled internally and all strains bearing a chaperone overexpression vector are therefore competed against the same genotype bearing an empty vector.

A vector bearing a reporter cassette that is likewise flanked by recombination sites is then co-transfected with a vector expressing the cognate site-specific recombinase (Cre or Flp, respectively).

WT Scl and Lmo2 cDNAs were tagged in their 5′ end with an oligonucleotide encoding avidin-TEV-FLAG (Driegen et al., 2005) and cloned downstream of the EF1α promoter in a vector bearing a neomycin- or zeocin-resistance marker (pEF1αbio-SCL and pEF1αbio-LMO2).

The lentiviral vector used was either the SIN-EF1a vector described by [14] and obtained from Addgene, a lentiviral vector bearing a PGK promoter for Sox2 as shown in Figure 1 (Sox2 expression appeared more robust from the PGK vector).

The L3.6pl cells were stably transduced with a retroviral vector bearing a dual reporter gene, termed GL, which is a tandem fusion of the green fluorescence protein and firefly luciferase genes.

To resolve this, we transfected the Fadd−/− cells with a second vector bearing a doxycycline-inducible MLKL construct.

Genomic DNA from L. kluyveri was digested to completion with MboI (a 4-cutter restriction enzyme) and ligated into an ARS-less vector bearing a URA3 marker.

In particular, expression of a dominant negative AP-2 protein in mammary cancer cells was shown to result in the inhibition of the transcription from a reporter vector bearing a 6-kb fragment of the ERBB2 promoter [ 13].

To determine whether the reduced levels of sirtuin 3 were responsible for the increased levels of acetylated p53 in peroxide-treated cells, we overexpressed sirtuin 3 in these cells using an adenoviral vector bearing a sirtuin 3 construct (adSirt3).