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Polarizations of the 'pump' and the 'Stokes' beams were set using an anti-reflection coated Glan-Thompson calcite polarizer (Newport model 10GT04AR.16) located before the microscope laser scanner.
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The beam size at the sample was set using the spectral bandwidth software parameter of 2, which produced a beam ∼3 mm wide and ∼7 mm tall.
The electron beam was set at 1 kV for all imaging.
The effective energy of the X-ray beam was set to 40 keV and 0.5 mA.
The planning parameters were set up using Varian standard scale in the Eclipse TPS (version 11.0.21) with Varian Clinac iX 6 MV X-ray beams (Varian Medical Systems, Palo Alto, CA, USA).
The beam voltage and the beam current were set to 700 V and 70 mA, respectively.
91 common steps were identified for external beam radiation therapy, 35 safety barriers were set.
The beam diameter was set to13 pixels, and the bleach time was 7 ms per pixel.
Beam hardening correction was set to 60%%.
Laser beams were later used for the same purpose.
An argon laser at 488 nm was used for excitation with a 515 nm beam splitter and the spectral detector was set between 510 and 550 nm.
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