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The laser beams were overlaid using three dichroic mirrors and focused into the sample.
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Images were overlaid using Adobe Photoshop.
The green and red images were overlaid using ImageJ.
Fluorescent micrographs were overlaid using GIMP digital software (version 2.6.1).
Images were overlaid using Adobe Photoshop CS2 or ACD Systems Canvas.
Datasets associated with the TGFβ pathway were overlaid using the IPA Pathway Designer.
Images were overlaid using Adobe Photoshop 7.0 (Adobe, Toronto, ON, Canada).
Appropriate light and electron micrographs, from the matching z position, were overlaid using Adobe Photoshop Elements.
Gels were overlaid using Phoretix 2-D Expression software (Nonlinear Dynamics, Durham, NC).
Information on historical fishing effort, substrate distributions and current and previous closure boundaries was overlaid using GIS to locate suitable monitoring sites.
The sample was overlaid using a modified EZYprep deposition method [ 21].
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