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The beam was reflected from a notch filter (Semrock, Inc., Rochester, New York) at near-normal incidence before being directed into the upright microscope (Eclipse E400, Nikon Instruments Inc., Melville, New York).
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One beam is reflected from the object that is having its picture taken; the other is a "clean" reference beam.
As shown in Figure 1, an object image is usually magnified by using an objective lens in the DHM system, and the enlarged object beam is reflected from front and back surfaces of an optical window glass.
The cantilever deflection, measured by a laser beam, is reflected from the gold-coated cantilever surface, which permits force/distance curves of single cells to be measured.
The incident beams are reflected from the reference mirror and the sample surface, respectively.
A part of the incident beam was reflected at the interface of glass substrate and the culture medium to give a reflection beam (R1).
A part of transmitted beam was reflected at the boundary between the cell and the culture medium and generated another reflection beam (R2).
Monochromatic p-polarized light from a He-Ne laser beam (λ = 633 nm) was reflected from the backside of the gold-coated glass slide.
One beam, the reference beam, is reflected off of a mirror to hit the emulsion straight-on; the other is targeted to hit the object before reflecting onto the plate.
The expanded beam is reflected by a mirror M1 and the beam diameter is scaled down by a second beam expander formed by L3 and L4.
An incident light beam is reflected into several diffraction orders by the gratings.
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