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For CO PLIF imaging the 230 nm laser beam was directed into the reactor and shaped into a ∼5 mm high vertical sheet using two cylindrical lenses of focal lengths f = +500 mm and f = +300 mm.
The laser beam was directed into the entrance port of integrating sphere 1, whose exit port was coupled with the entrance port of integrating sphere 2; the sample was situated between the two integrating spheres, as seen in Fig. 1.
A home built optical transfection setup was used for the optical transfection experiment as shown in Fig. 2. A collimated laser beam was directed into a combination of a half-wave plate and an optical isolator (Laser2000, UK, I-80-2), wereh were used to eliminate the back reflection from the beam path.
The beam was directed into a microscope (Eclipse T i, Nikon), with a high reflecting mirror for 800 nm as shown, to direct the beam to the objective and split the laser beam from the image for viewing on the CCD camera (DFK41BU02, The Imaging Source).
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If a weaker probe laser beam is directed into the sample along the opposite direction, it will interact with those atoms that have the appropriate Doppler shift to be resonant with the light.
It utilizes multiple reflection by a conical cavity surface when a small-diameter laser beam is directed into the cavity.
EISCAT radar beam was directed along the geomagnetic field line.
The central beam was directed on the symphysis pubis.
The expanded and collimated beam was directed through an interferometer.
The beam was directed to a 0.5 NA, 20x air objective (Nikon).
Laser beam was directed perpendicularly to the surface of the iris and retina.
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