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Beams were organized linearly along one horizontal axis in 5-cm increments and total beam breaks were used as the dependent variable.
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Total number of beam breaks was used to assess general locomotor activity.
For horizontal activity, consecutive beam breaks were recorded from two separate photocell beams.
Briefly, activity (total number of beam breaks in the lower level), mobility (at least two consecutive beam breaks in the lower level) and rearing (number of rearing beam breaks) were measured.
Beam breaks were interrupted by the presence of running wheels, so beam-break activity data could not be simultaneously assessed.
All photocell beam breaks were recorded by the computer with 55 ms accuracy.
Total beam breaks were recorded in 5 min time-bins over a period of 15 min.
At the beginning of the test session, animals were placed in the middle of the test cage, and locomotor activity (in the form in infrared beam breaks) was recorded for 30 minutes using a Visual Basics-based software (ActivityX16, provided by Laurent Monney, Institute of Physiology, University of Fribourg, Switzerland).
The number of beam breaks was recorded over a 60-min period, separated into 5-min time bins.
An automated beam-break system was used to assess locomotor activity over a 2-h period.
Extra steel beams were used to reinforce the vulnerable side.
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