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The reaction was then dumped into a beaker of ice water (50 ml) to quench the NaN3, transferred to a separatory funnel and extracted with EtOAc Et2O (1:2, vol/vol; 4 × 25 ml).
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And here's a sock, and a half-drunk Starbucks plastic beaker of iced coffee.
Place 150 ml sucrose saline, and empty 50 ml beaker on ice.
Cut these with fine scissors and remove brain to 50 ml beaker on ice.
The sample was homogenized using an AVESTIN Emulsiflex-C3 system, and the lysate was collected in a beaker on ice.
Once skull is removed, pour sucrose saline over brain into 50 ml beaker on ice to chill brain.
Even if you're in a place with no electricity, you can still do it with a Bunsen burner, a beaker of water with ice, another with hot water and someone with a thermometer who checks on everything.
Briefly, a solution of NaBH4 (0.01 M) and a separate solution of AgNO3 (0.01 M) were prepared, and 0.2-mg trisodium citrate was weighed in a beaker containing 44 ml of ice cooled DD water in an ice bath.
The processed tissue was filtered through eight sheets of cheesecloth and two sheets of Miracloth (Calbiochem, San Diego CA, USA) into a 1-liter beaker in ice.
Exact experimental procedures are as follows: 97 mL of distilled water was placed in a 250 mL glass beaker in an ice bath.
A lot of ice.
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