Sentence examples for beads were washed using from inspiring English sources

The beads were washed using RIPA buffer [58]and protein-DNA complexes were eluted using ChIP elution buffer [59].

The beads were washed using 10 vol of Borate Buffer (Sodium Borate pH 9.0, 0.2 mM) twice.

Beads were washed using a vacuum manifold and biotinylated reporter antibodies were added, followed by streptavidin-phycoerythrin.

After vortexing the mixture for 10 min at room temperature, the beads were washed using the PyroMark Q96 Vacuum Workstation (Qiagen).

Beads were washed using binding buffer with 1 M KCl, and MutH was eluted with 250 mM imidazole in binding buffer.

Bromopropionic acid (89 μL, 0.95 mmol) was then added to the vial and the vial was cooled to −20 °C in the freezer for 15 min. Beads were washed using DMF, DCM and then THF, respectively, for 5 times each.

After 60 minutes of incubation, the filter plates containing the bead-immobilized antibody-antigen complexes were washed using a BioMek robotic workstation with a vacuum manifold.

The resulting supernatant was then pre-cleared with 100 μl RSB100-buffer/protein-A-sepharose beads (50% (v/v), these beads were washed and used as controls).

After centrifuging, beads were washed three times using 0·1% PBS-T, bound protein eluted using non-reducing loading buffer and eluate analysed by SDS-PAGE.

Next, the beads were washed two times using 500 μl PBS.

Beads were washed four times using lysis buffer without Triton-X-100 and protease inhibitors.