Sentence examples for beads were then resolved from inspiring English sources
Proteins eluted from beads were then resolved on SDS-PAGE and analysed by mass spectroscopy (see Materials and Methods for details).
Retained proteins were eluted by directly boiling beads in SDS-PAGE loading buffer; beads were then removed, and samples resolved by SDS-PAGE.
Beads were then removed from the devices by centrifuging the devices upside down to push the beads out of microwells and flushing them out into an Eppendorf tube.
Any conflicting interpretations are then resolved.
The Sepharose beads were then washed five times with the same buffer before the proteins were resolved on a 15% SDS polyacrylamide gel (SDS-PAGE), transferred to a PVDF membrane, and detected by immunoblotting with an anti-Flag antibody (Sigma).
Beads were then washed with Mg2+ lysis/wash buffer and bound material was eluted with 25 µl 2× Laemmli sample buffer, boiled for 5 min, resolved by SDS PAGE and immunoblotted using mouse anti-RhoA antibody (3 µg/ml).
The beads were then thoroughly washed with lysis buffer containing 20 mM imidazole and resuspended in 5X SDS sample buffer followed by boiling for 15 min. The samples were resolved on SDS-PAGE followed by autoradiography.
The beads were then washed three times with TBST.
The cell lysates and beads were then incubated for an hour to capture the released mRNA molecules onto barcoded beads.
