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The beads were subsequently collected by centrifugation and washed with RIPA buffer containing protease inhibitor (Roche).
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Precleared protein extracts (200 500 μg) were incubated using the indicated antibodies or control IgG antibody at 4°C overnight and protein complexes were subsequently collected by incubation with Gammabind G Sepharose beads (GE Healthcare) for 1 h at 4°C.
The protein-antibody complexes were subsequently collected by adding protein A-agarose beads and incubated for 2 hrs at 4°C.
Saliva and blood samples were subsequently collected.
Blood samples were subsequently collected monthly.
Blood plasma was subsequently collected.
Beads were subsequently washed four times with a lysis buffer.
Beads were subsequently washed four times with the lysis buffer.
The beads were subsequently suspended in 1x PBS (0.8 mL) and stored at 4°C before use.
Beads were subsequently centrifuged and washed extensively with binding buffer.
The beads were subsequently washed and eluted for surface protein.
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