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DNA containing beads were sequenced using the LR70 sequencing kit on a full standard Pico Titre Plate (PTP).
After bead recovery and enrichment, the beads were sequenced using a GS LR70 sequencing kit (Roche) and the appropriate instrument run protocol.
4 nM of beads were sequenced using paired-end 250 nucleotide reads on Illumina MiSeq.
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After cleavage of the compounds from each bead using CNBr, the compounds were sequenced using MALDI-TOF MS/MS (Table 1).
The enriched beads were annealed with sequencing primers, and the amplified fragments were sequenced using the GS Junior Benchtop System (Roche).
Enriched DNA Capture beads were sequenced on the 454 (Roche Applied Science) using FLX Titanium chemistry per the manufacturer's instructions on a full 2-region slide for the fragment library and one region of a 2-region slide for each of the three paired-end libraries.
Dr. Brenner has invented a method for sequencing all the genes on a set of beads in parallel, meaning that all the beads are sequenced at the same time.
Following emulsion PCR, DNA bound beads were isolated and sequenced using long-read (LR) sequencing kits.
PCR products were cycle sequenced using BigDye reaction kits and the PCR primers, and unincorporated dye terminators were removed using CleanSEQ magnetic beads (Agencourt, Beckman Coulter).
After that, the double-stranded cDNA fragments are captured by streptavidin-coupled magnetic beads, and sequenced using multiplexed paired end sequencing on Illumina (Fig. 1).
The PCR product was isolated by sepharose beads, washed, and sequenced using specific sequencing primers (EpigenDx, Inc) on the Q24 pyrosequencer (Qiagen).
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