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The proteins bound to the beads were resolved in 10% SDS-PAGE and blotted to a nylon filter film.
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The protein bound to the microcystin beads were resolved by SDS-PAGE gels and detected by Western blotting to identify the components precipitated on the beads.
Samples of lysate preincubation (total protein) and postincubation with the GST-PAK-CRIB beads (active protein) were resolved in 12% polyacrylamide gels, transferred to PVDF membranes, and immunoblotted with anti-Rac1 or anti-Cdc42 antibodies as described above.
After washing the beads with lysis buffer, precipitated proteins were resolved in sample buffer, boiled, separated by 4%, 3 8% or 4 12% gradient NuPAGE pre-cast gels (Invitrogen), transferred onto PVDF membranes, and analysed with the indicated primary and secondary Abs.
Multiplex were resolved in a Bio-Plex 100 (Bio-Rad) bead sorter.
Then the beads were centrifuged and washed four times with cell lysis buffer, and the immunocomplexes were resolved in a western blot.
Disagreements were resolved in consensus.
All were resolved in short order.
Discrepancies were resolved in consensus.
Discordances were resolved in consensus.
These bead populations could be resolved in the fluorescence channels of the flow cytometer.
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