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Beads were maintained in homogenous suspension overnight at 4°C using a rotary wheel that supports 'end-over-end' mixing.
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(A ) I. A cohort of cells with cell-wall attached beads is maintained in the magnetized column (magnet 1) and harvested at set time points (column fraction) when also a fraction with mainly daughter cells is collected (column effluent, mix 3).
By using minimal medium, yeast cells inside the alginate beads were maintained, and its growth was kept at its minimal.
Cells were maintained in suspension by the addition of a 3-mm glass bead in each well and constant shaking at 220 rpm.
Control cells were maintained in SF-MEM alone.
Animals were maintained in conventional housing facilities.
Yeast cells were maintained in YEPD medium.
Cells were maintained in T-25 flasks.
Control cultures were maintained in SF-MEM alone.
Cells were maintained in DMEM/F12 (1∶1) medium (Mediatech Inc).
Undifferentiated hMSCs were maintained in control medium.
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