Sentence examples for beads were maintained for from inspiring English sources
The beads were maintained for up to 14 days with Chondrocyte Differentiation Media (Cambrex CC-3225) and seeded in a 12-well plate at a density of 1 × 105 cells/well.
By using minimal medium, yeast cells inside the alginate beads were maintained, and its growth was kept at its minimal.
The beads are stored at 4 °C, and their Sav binding capacity is maintained for several years (>5).
The optimized concentration of 15%% (w/v) was maintained for Eri as well as Tasar fibroin solutions for producing nanofibrous scaffolds without any beads or spraying.
After reaching a defined force, here 10 nanoNewton (nN), the contact is maintained for 5 s before retracting the cantilever and recording its deflection, caused by the occurring adhesive forces between bead and bacterium (Fig. 1b).
After the cells were maintained at 4°C for 15 minutes, 100 µl of the bead solution (InlA-beads for MDCK and Caco-2 and fibronectin-beads for NIH 3T3) was deposited into the petri dish and the dish was centrifuged at 3600 rpm for 3 minutes.
After the cells were maintained at 4°C for 15 minutes, 100 µl of either the Alexa488/InlA-beads or FITC/InlA-beads suspension, which was at bead density of ∼1×109 beads/ml, was deposited into the petri dish (this volume was selected because this amount of beads was sufficient to cover the entire area of the bottom of the petri dish) and the dishes were centrifuged at 3600 rpm for 3 minutes.
After denaturing at 75°C for 10 minutes, the mixture was maintained at 37°C for 10 min. MagPrep® Streptavidin Beads (Novagen) were added to the mixture to remove the hybrids and the free probes.
