The beads were isolated using a magnet, washed, mounted on a glass slide and imaged by confocal microscopy.
The cells captured by the magnetic beads were isolated using MagSweeper (Illumina, Hayward, CA, USA) to harvest the beads from the cell suspension.
Cells attached to beads were isolated using a magnetic particle concentrator (Dynal, Merseyside, UK) and cultured for 6 hours at 37°C.
Samples were washed five times with lysis buffer after which, RNA bound to beads was isolated using Trizol LS according to manufacturer's instructions with the following modifications: after RNA precipitation, RNA was DNase treated for 30 min at 37°C and then extracted with Acid:Phenol CHCl3 (Ambion) before a second precipitation with 100% Ethanol.
CD14+ monocytes were isolated using magnetic bead separation with anti-CD14 antibody-labeled magnetic beads and iron-based MidimacsLS colums (MiltenyiBiotec).
Dopaminergic synaptosomes were isolated using magnetic beads conjugated to anti-rabbit IgG (Miltenyi Biotech) after capturing them using a rabbit anti-DAT antibody (Chemicon).
CD11c+ DCs (4×105/well) were isolated using MACS beads (Miltenyi Biotec) and incubated with 1 mg/ml OVA for 3 h, then co-cultured with DO11.10 CD8+ T KJ1-26+CD8+ KJ1-26+CD8+105/well) for 24 h, and the expression of cellson DO11.10 CD8+ T cells was examined.
Naive T cells (i.e., nCD4+CD25+, nCD4+CD25−, nCD8+CD25+, nCD8+CD25−) from mock-infected mice, and effector T cells from HSV-IL-2-infected mice (i.e., effCD4+CD25+, effCD4+CD25−, effCD8+CD25+, effCD8+CD25−) were isolated using magnetic beads as described by the manufacturer (Miltenyi Biotec, Auburn, CA).
CD4+CD25+, CD4+CD25−, CD8+CD25+, and CD8+CD25− T cells were isolated using magnetic beads from naive BALB/c mice (naive T cell, nT cell) or BALB/c mice infected ocularly with HSV-IL-2 (effector T cell, effT cell) and the cells injected intraperitoneally into BALB/c-SCID recipient mice.
The HMW-MAA-positive cells were isolated using immunomagnetic beads.
CD4+ and CD8+ subsets were isolated using Miltenyi beads and protocols provided by the manufacturer (Miltenyi, Auburn, CA).
