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The Alexa488/InlA beads were imaged using a 450 490 nm excitation filter and a 505 nm emission filter, and the FITC/InlA-beads were imaged using two different excitation filters, 470 nm and 430 nm, and a 505 nm emission filter for both excitation wavelengths.

Fluorescent beads were imaged using a 40× objective (LD C-Apochromat 40×/1.1 W korr, pixel size x, y 0.131203 µm), where frames were captured every 4 seconds one hundred times similar to previously described conditions (Satpute-Krishnan et al., 2006) and re-analyzed (Vermot et al., 2008).

Briefly, bisulfite converted tumor DNA was hybridized to the bead array as described previously, and bead arrays were imaged using Illumina Cancer Panel Reader software.

The SAMs were imaged using an Illumina BeadArray Reader, which measures the fluorescence intensity at each addressed bead location.

The arrays were imaged using an Illumina BeadArray™ Reader, which measures the fluorescence intensity at each addressed bead location.

A single-base extension reaction was carried out and the fluorescently stained chip was imaged using the Illumina Bead Array Reader and the Bead Scan Software (Illumina, USA).

Beads were imaged before and after photo-bleaching using a spinning disk microscope (Visitron).

Beads were imaged with an Olympus IX70 fluorescence microscope, and the fluorescence intensities of the beads at 509 nm were measured using a Tecan Infinite M1000 fluorescent microplate reader with an excitation wavelength of 488 nm.

After 30 minutes the beads were imaged again.

An electron microscopy modality in which proteins labelled by using antibodies that are tagged with small gold beads are imaged, allowing their localization.

Beads were then imaged using an Olympus Fluoview confocal microscope.