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Five percent of the beads were frozen for SDS PAGE analysis after the second wash.
The beads were incubated with the lysate at 4°C for 4 hr and washed twice with 10× volume of lysis buffer for 5 min. Five percent of the beads were frozen for SDS-PAGE analysis after the second wash.
Similar(58)
It's been frozen for six years.
Hiring had been frozen for years.
Swords have been frozen for the duration.
Public pay could be frozen for longer.
These can be frozen for several months.
They can be frozen for several months.
Infected cells were harvested using glass beads and centrifuged at 7,500 rpm for 10 min. Pellets were frozen using liquid nitrogen and stored at −80°C.
Then, 250 uL of acid-washed glass beads were added to each well and the cells were frozen at −80°C.
The needles were frozen overnight at −20 °C then freeze-dried for 24 48 h.
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