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Lengths of pseudo-bonds between Cα beads were fixed at the average values of the two structures.
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Small agarose cubes containing the immobilized beads were fixed overnight at 4°C using 2% glutaraldehyde in 0.1 M cacodylate buffer at pH 7.4.
For visualisation of exosomes following immunomagnetic purification, loaded beads were fixed for 1 hour at 4°C in 1.6% glutaraldehyde in phosphate Sörensen buffer 0.1 M, pH 7.3, and washed 3 × 20 min in Phosphate buffer.
Beads were fixed in 4% PFA, dehydrated, embedded in paraffin and sectioned (5 µm).
Silica beads were fixed in space while all the protein atoms were free to move in all directions.
After passing and washing the liposomes on G-25 fine beads were fixed with 2% glutaraldehyde in 0.1 M cacodylate buffer (pH 7.4) (Sorensen buffer) containing 6 mM CaCl2, for 4 h, at 4°C.
Previously drawn beads were displayed throughout the trial, to reduce working memory demands, and the sequence of drawn beads was fixed across all participants.
Cells were fixed at 4 DIV.
The chain length is fixed at 12 beads at every volume fraction (including 1/1, 1/4, 1/6, 1/14, and 1/20).
Thus, the aqueous solution for the bead milling of Cs0.33WO3 coarse powder was fixed at pH 8 by adding potassium hydroxide in deionized water.
The optimum pH for removal of atrazine was fixed at 6.0 ± 0.6 for AgNPs beads.
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