Sentence examples for beads were eluted using from inspiring English sources

The beads were eluted using 1% SDS and 0.1 ℳ sodium bicarbonate solution.

Finally, any proteins bound to the beads were eluted using boiled 4× Laemmli buffer, and recovered by centrifugation (15,600 g for 3 minutes).

FLAG BRI1 protein bound to the beads was eluted using sample buffer and detected by immunoblotting with anti-His6 antibodies.

The beads were washed using RIPA buffer [58]and protein-DNA complexes were eluted using ChIP elution buffer [59].

For mass spectrometry proteomic analysis, samples were eluted using on-bead trypsin digestion as described previously and submitted to the University of California, Davis, Genome Center Proteomics Core.

His6-tagged proteins remaining on the beads (i.e. cleaved CPD-His6) were eluted using high imidazole buffer [500 mM NaCl, 50 mM Tris-HCl, pH 7.5, 175 mM imidazole, 10% glycerol] in 300 500 µL volumes.

For elution, following binding STAT3 using inS3-54-conjugated beads as described above, the protein bead complex were eluted using vehicle control, 300 μM inS3-54 or the irrelevant compound in binding buffer containing 20% DMSO.

After extensive washing, proteins were eluted using pH 2.5 glycine or trypsin was added directly to a small aliquot of DPSL beads.

Pelleted beads were washed three times with 1 ml ice-cold TBS containing 0.1% Tween 20 and bound proteins were eluted using SDS PAGE sample buffer.

The protein DNA complex was eluted using 200  μl of elution buffer from the beads.

The genomic DNA-beads-antibody complex was washed and methylated DNA was eluted using the Dynal Magnetic Particle Concentrator.