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As the XRF measurements for the Magoro Hill beads were done using a portable instrument, beads with uranium content slightly lower than 100 ppm were not excluded from the Khami group based on their uranium content.
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Preparation of beads was done using a modification of the method of Yamamoto et al [17].
Proper alignment of the excitation beams on a polystyrene bead is done using a CARS image such as the one presented in Fig. 6(b).
The analyses of the bead median fluorescence intensities were done using the Bio-Plex Manager software (version 4.1.1).
Determination of bead activity was done using a modification of the method of Yamamoto et al [17].
Bead counting was done using a Bio-Rad TC10 automated cell counter (Bio-Rad Laboratories).
After ligation, purification of amplicon libraries was performed with AMPure beads, and assessment of library quality was done using the Agilent Bioanalyzer with High Sensitivity DNA Chip.
Positive selection of DC was done using CD11c beads (clone N418, Miltenyi Biotec, B.Gladbach, Germany), whereas negative selection/enrichment was done on the CD90 T-cell marker (Thy1.2, 30.H12, Miltenyi Biotec).
Sample cleanup was done using AMPure SPRI beads.
Quantification of targeted protein mediators (cytokines, chemokines, and acute-phase proteins involved in inflammation) was done using validated Luminex bead-based multiplexing assays according to manufacturer's instructions (Luminex, Austin, TX, USA).
The cytokine bead assay was performed according to the manufacturer's specifications and data analysis was done using BD FACSDiva software.
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