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Spherical beads were created by dispensing droplets of alginate cell suspension from the tip of an 18-gauge needle into a bath of 120 mM CaCl2, 10 mM HEPES, 0.01% Tween 80 and 150 mM NaCl followed by gelation for 20 minutes.
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The homogeneous column was packed with glass beads (diameter 500 600 μm), and physical heterogeneities were created by sequential layering or mixing two sizes of glass bead (500 600 μm and 300 400 μm).
These were created by glaciation.
More complicated chains can be created by threading beads onto the wire before knitting, inserting beads into the chain as its formed, or by using multiple wire threads at the same time.
X-aptamers are created by a bead-based pseudorandom process incorporating encoded specialty 5-X-dU bases (labeled as W, X, Y, or Z).
Multiplex assays can be created by mixing bead sets with different conjugated capture antibodies to simultaneously test for many (up to 50 or more) analytes in a single clinical sample (Elshal and McCoy, 2006).
The roughness was created by gluing glass beads of known mesh size on both inner faces of the two plates.
They are created by humans.
It was created by Adam Fleischman.
Drizzt was created by happenstance.
Mean pixel intensities by bead type, were created using BeadStudio v3.1 and processed with the R/beadarray package [ 58].
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