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Polymer beads were constructed using polymerization of styrene monomer and divinylbenzene.
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The six samples, each with an RNA Integrity Number (RIN) of over 6, were sent to the Otago Genomics and Bioinformatics Facility, where mRNA was isolated using oligo-dT magnetic beads, and cDNA libraries were constructed using the Illumina TruSeq Stranded mRNA Sample Preparation Kit.
The polymer beads which possessed improved physical properties were constructed using polymerization of styrene monomer added with divinylbenzene.
Affinity chromatography columns were constructed using agarose beads as the solid phase to which Sambucus nigra lectin (Vector Laboratories, 3 mg lectin/ml gel) had been covalently bound.
Five replicate experimental columns were constructed using zeolite, scoria, riversand and polymeric glass beads, and different flow-through rates were achieved using restricted outlets.
Briefly, mRNA was purified using oligo-dT magnetic beads, cDNA was synthesized using random hexamer primers, and sequencing libraries were constructed using the cDNA.
Finally, the cDNA libraries were constructed using PCR amplification and products were purified using AMPure XP beads.
Genome libraries were constructed using eight biotin labeled probes and a selective hybridization with streptavidin coated bead method [ 21- 23].
Phylogenetic trees were constructed using MEGA3 [73].
Kymographs were constructed using Metamorph 7.1.4 software.
Neighbor-joining trees were constructed using NEIGHBOR.
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