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After incubation, beads were collected using a magnetic stand (Invitrogen).

The immunoprecipitated complex-magnetic beads were collected using magnetic separator and washed according to manufacturer's instruction.

Beads were collected using a minicentrifuge at 6.000rpm for 45s and the supernatant was saved for RNA extraction.

The beads were collected using a magnetic stand, washed in CHAPS buffer containing 120 mM NaCl and 40 mM HEPES, and then in CHAPS buffer containing 200 mM NaCl and 60 mM HEPES.

Next, the beads were collected using a magnet stand, and the supernatant was removed.

Beads were collected using a magnetic particle concentrator followed by several washes with 1% BSA in HBSS.

The magnetic beads (with the captured vascular fragments) were collected using a magnetic rack, washed extensively after which RNA was prepared from the captured cells using the Quiagen RNeasy Mini Kit (Quiagen, Hilden, Germany).

The PCR products were collected using streptavidin magnetic beads (Dynabeads M-280 Streptavidin, Invitrogen).

CD19+ and CD19− cell populations were collected using the magnetic beads system described earlier (see Materials and Methods).

Complexes were collected using Protein-A magnetic beads (Pierce) pre-blocked with BSA (New England Biolabs) and transfer RNA (Roche), then washed and eluted.

Antibody-chromatin complexes were collected using protein A agarose beads.