Sentence examples for beads in a batch from inspiring English sources
The first method was purification using Ni Sepharose high performance beads in a batch approach.
The cell lysate was spun at 20,000 g and then GST-PsGA2OX1 was extracted from the supernatant using a bed volume of 2 mL of beads in batch mode.
The solubilized CD81 receptor was adsorbed to rho-1D4-Sepharose beads in batch mode (binding capacity corresponding to 1 mg rhodopsin/ml of beads) with slow agitation for 2 4 h at 4°C.
P. pastoris strain X-33 was cultivated in shake flasks under four different bioprocess conditions (Table 1): excess glycerol or glucose (batch culture conditions; these cells were harvested during exponential growth); limiting glucose (using slow glucose-releasing silica disks or feed beads in fed-batch mode, [ 21, 22]); and periodic methanol addition (methanol induction conditions).
White beads in A correspond to control beads without lycopene.
Yet, it is possible to produce beads, although the last beads prepared out of a batch tend to appear brittle.
The second supernatant was diluted with an equal volume of lysis buffer without salt and then incubated at 4°C with Ni-NTA agarose beads (Qiagen) in batch.
The supernatant was bound to 20 ml GST sepharose 4B beads (GE Healthcare) in batch, rotating for 2 hr, at 4°C.
The 5' capped RNAs were purified as previously described using GST-eIF4EK119A recombinant protein bound to glutathione-agarose beads in microfuge tubes by batch purification [20], [64].
In the kitchen, a batch of chickens had come in.
