Your English writing platform
Discover LudwigExact(12)
We then conducted a second experiment in which rhizoboxes were free of beads (data not shown).
Similar results were obtained in presence of GST-9G8 and GST-SRp20-coupled Sepharose beads (data not shown).
We observed that at a concentration of 0.2% SDS (50 mM Tris, pH = 8.0) incubation at 25°C is already sufficient to elute rabbit polyclonal Ig from protein A sepharose beads (data not shown).
We found that from a 106-fold dilution an average of 7.2±1.05 beads were isolated in 10 independent experiments, which is very close to the expected number of 8 beads (data not shown).
A pilot experiment confirmed that the VP51A-FLAG proteins could be efficiently precipitated by the anti-FLAG antibody, and binding specificity of VP51A-FLAG with anti-FLAG M2 affinity gel was reconfirmed by subjecting VP51A-FLAG protein to reaction with anti-HA antibody conjugated beads (data not shown).
Capture of this protein by SptPD441A was blocked by preincubating the brain extract with vanadate prior to addition of the beads (data not shown).
Similar(48)
Live imaging of mitf GAL4-UAS:mitf GAL4-UASants (pu.1 mCherryibited no recruitmorphantselanoblasts to the impu.1ted bead (data not shown).
The higher entrapment efficiency observed with the coated formulations was further investigated using optical microscopy (Nikon model TiU coupled with Photometric Coolsnap EZ 20 MHz monochrome camera) to investigate how the beads dried (data not shown).
In the control experiment no binding of BRCA1 230-534 was observed to glutathione sepharose beads alone (data not shown).
Quantitation of the number of macrophages per microscopic field indicated an ∼8-fold increase in macrophage invasion when tumor cells were present in the collagen compared to beads alone (data not shown).
Interexperiment instrument stability was assessed by CS&T beads (BD) (data not shown).
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com